His art investigates various natural and emotional phenomena, often using technological and mechanical tools to create his works. Through a thorough study of human cognitive faculties, Piccolo's art analyses the perceptive aspects of the natural world. His artwork investigates natural, physical, biological, and scientific phenomena which are the basis of living, employing their emotional aspect as a tool. In order to induce such a peculiar resonance phenomenon , most of the artist's works combine two complementary and inseparable aspects: they are at the same time sculptures and machines, forms and processes. According to Donato Piccolo, this hybrid character constitutes the actual nature of «holistic art»: an art whose essential function is to explore "the incomprehensible mystery of the visible world".
Donato Piccolo is one of the most remarkable artists of his generation, both in Italy and on the international scene.
His work has been exhibited in numerous national and international galleries and institutions. Among its main exhibitions include: MAXXI Museum, Rome Italy; International Venice Biennale (editions: 2011, 2016); Hermitage Museum in St Petersburg, Himalayas Art Museum, Shanghai, China; Macro Museum, Rome, Italy, Stieglitz Academy, in St Petersburg, Media Art Center, NY, USA, Museum of Academy of Art, Hermitage in San Pietroburg, Russian; Museum of Cuba, Center Desarollo, Palazzo delle Esposizioni in Rome, Italy; Festival dei due Mondi (2009, 2016), Spoleto italia; Macro Museum Rome ; Foundation Francès, Senlis, France; Huyndai Beyond Museum, Seoul; Museum Biedermann, Donauschingen, Germany; Musma museum of Matera, Italy; Georg-Kolbe Museum, Berlin; Boghossian Foundation, Brussels, Belgium; Arnaldo Pomodoro Foundation, Milan, Italy; Stadtgalerie Kiel; Space Gallery, Bratislava, Czech Rep.; MACUF, Museo de Arte Contemporáneo Gas Natural Fenosa, Coruña; Centre Saint-Benin, Aosta; Festival of Two Worlds, , Spoleto, Italy ; Wood Street Gallery, Pittsburgh USA